Protein_Domain

Part:BBa_K1422501:Design

Designed by: Casey Martin   Group: iGEM14_Purdue   (2014-10-13)


Zea mays nicotianamine synthase (corn NAS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 334
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 334
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Bacillus subtilis. The 28th, 115th, and 140th amino acid sequences were changed to CTA from CTT. NAS can be produced from the genes nas1, nas2, nas3, nas4, nas5-1, nas5-2, and nas6. nas1 was selected because in a previous experiment it was able to, alone, produce NAS. Higuchi, K.; Suzuki, K.; Nakanishi, H.; Yamaguchi, H.; Nishizawa, N.K.; & Mori, S. (February 1999). Cloning of nicotianamine synthase genes, novel genes involved in the biosynthesis of phytosiderophores. Plant Physiology 119(2) 471-480. DOI: 10.1104/pp.119.2.471. http://www.plantphysiol.org/content/119/2/471/F1.expansion.html

Source

The original sequence came from the NCBI website. NCBI. (5 February, 2003). Zea mays ZmNAS1 mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/19911063

References